Have you ever wanted to know how many tumor cells were in each microliter of a blood sample? The newest CellEngine release expands the ability to calculate absolute cell counts, which determine the number of a specific cell type in a given volume of sample.
Creating a figure to communicate a gating hierarchy is a common task for data review and for sharing gating strategies with other scientists. These consist of a series of flow plots showing the gate positions and parent-child relationships between gated populations. CellEngine can now generate these plots automatically, making analysis even faster.
As a large contract research organization (CRO), CellCarta analyzes thousands of flow cytometry samples every month. CellCarta is leveraging automation tools such as CellEngine and laboratory information management systems (LIMS) for a seamless process.
This week at CYTO, we presented our poster examining the potential to identify cell types in CITE-seq data using hierarchical gating. Compared to clustering, gated populations have clear definitions based on specific marker expression levels. We compared cell type assignment by hierarchical gating vs. unbiased clustering.