Skip to content

Applications

The Art and Science of Phospho Flow: Capturing Cell Signals with Cytometry

Intracellular signaling cytometry uses antibodies that bind to specific epitopes on signaling proteins, usually to activated forms of the protein, to analyze signaling dynamics at a single-cell resolution. These data can be valuable for many kinds of studies, from basic research to clinical trials, but pose additional challenges compared to immunophenotyping. In this post, we discuss some common considerations when designing an experiment with signaling markers in the panel.

Eavesdropping on bacteria with CellEngine

No one is surprised to read that individuals coordinate their behavior and act as groups. We see collective behavior everywhere: from family gatherings, to corporate workplaces, and on the playing field. We also know that other animals act collectively. Birds flock, fish school, and wolf packs hunt. What might surprise us, however, is how ubiquitous the phenomenon is. Remarkably, coordinated, collective behavior can be seen in organisms as simple as bacteria, single-celled creatures without even a nucleus, much less a nervous system. Because they are so simple, bacteria can serve as a model organism for studying collective behavior. And because each individual is a single cell, CellEngine can be an important tool for analyzing that behavior.